【文獻標題】Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds

【作者】Guolin Cui，Shixun Zhong，Shifa Yang，et.al

【作者單位】山東農(nóng)業(yè)大學（Shandong Agricultural University）

【文獻中引用產(chǎn)品】

雞分泌型免疫球蛋白A（sIgA）elisa試劑盒

雞白細胞介素2（IL-2）ELISA試劑盒

【關鍵詞】Taishan Pinus massoniana pollen，polysaccharide，Subunit vaccine，Proteus mirabilis，Adjuvant，Effect

【DOI】http://dx.doi.org/10.1016/j.ijbiomac.2013.02.006

【影響因子(IF)】4.97

【出版期刊】《International Journal of Biological Macromolecules》

【產(chǎn)品原文引用】

2.4. Immunization

Threehundred1-day-oldchicks were randomlydividedintofive groups (n = 60 per group). Groups I–V were injected subcutaneously with 0.2 ml of WO-OMP vaccine, PP-OMP vaccine, TPPPS-OMP vaccine, OMP-only vaccine ａnd physiological saline, respectively, at 3,7 ａnd 12 days old. The final concentrations of OMP were 500 g/ml at the first vaccination, 1 mg/ml at the second vaccination ａnd 2 mg/ml at the third vaccination. Seven days after the third vaccination, 30 chicks from each group were challenged with 100 LD50 P. mirabilis strain Q1 by oral administration.

On days 3, 7, 14, 21, 28, 35, 42 ａnd 49 after the first vaccination,the peripheral blood ａnd duodenum were sampled for analysis.The antibody titers were detected using indirect enzyme-linked immunosorbent assay (ELISA), T-lymphocyte proliferation rates were detected with flow cytometry ａnd secreting-type IgA (SIgA)and interleukin-2 (IL-2) were measured using ELISA kits (Shanghai Hengyuan Bio-technology Co. Ltd.). The pathogenesis ａnd survival status of chicks were clinically examined daily for 14 successive days after challenge. The mortality, morbidity ａnd protective rates in each group were calculated as follows:

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